Corning Matrigel matrix is a solubilized basement membrane preparation extracted from₹ the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, inσcluding Laminin (a major component), Collagen IV, heparin sulfate↔ proteoglycans, entactin/nidogen, and a number of growth factors.
Quality • Mouse colonies are routinely screened for pathogens via Mouse Antib↓ody Production (MAP) testing • Extensive PCR testing is performed to screen for a number of pathog↑ens, including LDEV, to ensure strict control of raw materials us<ed during the manufacturing process • Tested and found negative for bacteria, fungi, and mycoplasma • Protein concentrations are determined by Lowry method • Endotoxin units are measured by Limulus Amoebocyte Lysate assay • Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C • Biological activity is determined for each lot using a ™neurite outgrowth assay. Chick dorsal root ganglia are plated on a≥ 1.0 mm layer of Corning Matrigel Matrix and must generateδ positive neurite outgrowth response after 48 hours without addition& of nerve growth factor
Source Engelbreth-Holm-Swarm Mouse Tumor
Preparation and Storage Store at -20°C. Avoid multiple freeze-thaws. Do not sto₹re in frost-free freezer. KEEP FROZEN.
Application • Cell Growth and Differentiation • Metabolism/Toxicology Studies • Invasion Assays • In Vitro and In Vivo Angiogenesis Assays • In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppr≥essed Mice
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010-64814275
010-64814275
2850669802